Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AMAb91033 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#AMAb91033, RRID:AB_2665775
- Product name
- Anti-GFAP
- Antibody type
- Monoclonal
- Description
- Monoclonal Antibody against Human GFAP, Clone ID: CL2713, Gene description: glial fibrillary acidic protein, Alternative Gene Names: FLJ45472, Validated applications: IHC, WB, Uniprot ID: P14136, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- CL2713
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references CD8 positive T-cells decrease neurogenesis and induce anxiety-like behaviour following hepatitis B vaccination
Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images.
Anti-Inflammatory Activity of Synaptamide in the Peripheral Nervous System in a Model of Sciatic Nerve Injury
Transient neurogenesis in ischemic cortex from Sox2(+) astrocytes.
Antinociceptive properties of losmapimod in two acute pain models in rats: behavioural analysis, immunohistochemistry, dose response, and comparison with usual analgesic drugs
Synaptamide Modulates Astroglial Activity in Mild Traumatic Brain Injury
Microglial TREM2 Mitigates Inflammatory Responses and Neuronal Apoptosis in Angiotensin II-Induced Hypertension in Middle-Aged Mice
Modulation of Hippocampal Astroglial Activity by Synaptamide in Rats with Neuropathic Pain
Learning-Dependent Dendritic Spine Plasticity Is Reduced in the Aged Mouse Cortex
Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue
Long-Term, Targeted Delivery of GDNF from Encapsulated Cells Is Neuroprotective and Reduces Seizures in the Pilocarpine Model of Epilepsy.
Lymphocyte activation gene 3 (Lag3) expression is increased in prion infections but does not modify disease progression
Zhou T, Gao Y, Wang Z, Dai C, Lei M, Liew A, Yan S, Yao Z, Hu D, Qi F
Brain Communications 2024;6(5)
Brain Communications 2024;6(5)
Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images.
Wernersson E, Gelali E, Girelli G, Wang S, Castillo D, Mattsson Langseth C, Verron Q, Nguyen HQ, Chattoraj S, Martinez Casals A, Blom H, Lundberg E, Nilsson M, Marti-Renom MA, Wu CT, Crosetto N, Bienko M
Nature methods 2024 Jul;21(7):1245-1256
Nature methods 2024 Jul;21(7):1245-1256
Anti-Inflammatory Activity of Synaptamide in the Peripheral Nervous System in a Model of Sciatic Nerve Injury
Starinets A, Tyrtyshnaia A, Manzhulo I
International Journal of Molecular Sciences 2023;24(7):6273
International Journal of Molecular Sciences 2023;24(7):6273
Transient neurogenesis in ischemic cortex from Sox2(+) astrocytes.
Yang JL, Fan H, Fu FF, Guo BL, Huang Y, Sun L, Wang WT, Xing JL, Hu XT, Ding YQ, Zhang K, Hu YZ, Wang YZ
Neural regeneration research 2023 Jul;18(7):1521-1526
Neural regeneration research 2023 Jul;18(7):1521-1526
Antinociceptive properties of losmapimod in two acute pain models in rats: behavioural analysis, immunohistochemistry, dose response, and comparison with usual analgesic drugs
Soued M, Hamdi L, Ben Rehouma M, Mazoit J, Benhamou D
BJA Open 2022;3
BJA Open 2022;3
Synaptamide Modulates Astroglial Activity in Mild Traumatic Brain Injury
Ponomarenko A, Tyrtyshnaia A, Ivashkevich D, Ermolenko E, Dyuizen I, Manzhulo I
Marine Drugs 2022;20(8):538
Marine Drugs 2022;20(8):538
Microglial TREM2 Mitigates Inflammatory Responses and Neuronal Apoptosis in Angiotensin II-Induced Hypertension in Middle-Aged Mice
Xu X, Du L, Jiang J, Yang M, Wang Z, Wang Y, Tang T, Fu X, Hao J
Frontiers in Aging Neuroscience 2021;13
Frontiers in Aging Neuroscience 2021;13
Modulation of Hippocampal Astroglial Activity by Synaptamide in Rats with Neuropathic Pain
Manzhulo I, Manzhulo O, Tyrtyshnaia A, Ponomarenko A, Konovalova S, Ermolenko E, Milkina E, Starinets A
Brain Sciences 2021;11(12):1561
Brain Sciences 2021;11(12):1561
Learning-Dependent Dendritic Spine Plasticity Is Reduced in the Aged Mouse Cortex
Huang L, Zhou H, Chen K, Chen X, Yang G
Frontiers in Neural Circuits 2020;14
Frontiers in Neural Circuits 2020;14
Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue
Rybachuk O, Kopach O, Pivneva T, Kyryk V
BIO-PROTOCOL 2019;9(4)
BIO-PROTOCOL 2019;9(4)
Long-Term, Targeted Delivery of GDNF from Encapsulated Cells Is Neuroprotective and Reduces Seizures in the Pilocarpine Model of Epilepsy.
Paolone G, Falcicchia C, Lovisari F, Kokaia M, Bell WJ, Fradet T, Barbieri M, Wahlberg LU, Emerich DF, Simonato M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Mar 13;39(11):2144-2156
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Mar 13;39(11):2144-2156
Lymphocyte activation gene 3 (Lag3) expression is increased in prion infections but does not modify disease progression
Liu Y, Sorce S, Nuvolone M, Domange J, Aguzzi A
Scientific Reports 2018;8(1)
Scientific Reports 2018;8(1)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GFAP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human cerebral cortex and tonsil tissues using AMAb91033 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol