Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- V3588 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant GFAP Antibody / Rabbit Monoclonal
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant GFAP antibody is suitable for use in Flow cytometry/Immunohistochemistry/Immunofluorescence/Western blot applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- ASTRO/1974R
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the recombinant GFAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human brain tissue with recombinant GFAP antibody. Predicted molecular weight ~50 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of frozen human cerebral cortex tissue with recombinant GFAP antibody (blue, clone ASTRO/1974R) and Histone H1 antibody (magenta, clone HH1/957).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human cerebellum tissue with recombinant GFAP antibody (clone ASTRO/1974R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed human T98G cells with recombinant GFAP antibody (clone ASTRO/1974R); Red=isotype control, Blue= recombinant GFAP antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant GFAP antibody (clone ASTRO/1974R) as confirmation of integrity and purity.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant GFAP antibody (clone ASTRO/1974R) as confirmation of integrity and purity.
