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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- MA1-19395 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GFAP Monoclonal Antibody (GF-02)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with intact GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system; it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- GF-02
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GFAP using a monoclonal antibody (Product # MA1-19395).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GFAP using a monoclonal antibody (Product # MA1-19395).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human GFAP using mouse monoclonal antibody GF-02 on lysates of Neuro 2a and HEK 293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-GFAP monoclonal antibody (Product # MA1-19395) followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GFAP at approximately 54kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis (reducing conditions) of GFAP in porcine brain lysate. Lane 1: immunostaining with anti-GFAP (GF-02) Monoclonal antibody (Product # MA1-19395); Lane 2: immunostaining with Isotype mouse IgM control (PFR-03).
