Antibody data
- Product number
- PA5-18598
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GFAP Polyclonal Antibody
- Provider product page
- Invitrogen Antibodies - PA5-18598
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine and rat based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Mouse Brain lysate using Product # PA5-18598 at a concentration of 0.1 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GFAP by a GFAP monoclonal antibody (Product # PA5-18598) at a concentration of 0.01 µg/mL. Human (A), Mouse (B) and (0.003 µg/mL) Rat (C) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Mouse Liver (Lane 3) and Mouse Ovary (Lane 4). The blot was probed with Anti-GFAP Polyclonal Antibody (Product # PA5-18598, 1:1000 dilution) and detected by chemiluminescence using Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25 µg/mL, 1:4000 dilution). A 50 kDa band corresponding to GFAP was observed in the Mouse and Rat brain, while it was not detected in Mouse Liver and Mouse Ovary.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELIZA analysis of GFAP concentration using a GFAP antibody (Product # PA5-18598) (1.5µg/ml) as the reporter with a capture rabbit antibody (5µg/ml).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELIZA analysis of GFAP concentration using a GFAP antibody (Product # PA5-18598) (1.5µg/mL) as the reporter with a capture rabbit antibody (5µg/mL).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GFAP in paraffin embedded Human Cerebellum tissue. Steamed antigen retrieval was performed with citrate buffer pH 6, followed by application of the GFAP antibody (Product # PA5-18598) (2µg/ml) and HRP-staining.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GFAP in a PFA-perfused cryosection of Human Hypothalamus tissue. Antigen retrieval was performed with citrate buffer pH 6 at 80C for 30min, followed by application of GFAP antibody (Product # PA5-18598) (0.01µg / ml) and HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemichal analysis of GFAP in Human Cerebellum using a GFAP polyclonal antibody (Product # PA5-18598) at a concentration of 2 µg/mL. The sample was paraffin embedded, and heat treated antigen retrieval was used to detect the target with HRP staining.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GFAP in a PFA-perfused cryosection of Human Hypothalamus tissue. Antigen retrieval was performed with citrate buffer pH 6 at 80C for 30 min, followed by application of GFAP antibody (Product # PA5-18598) (0.01µg / ml) and HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GFAP in Human Cortex using a GFAP monoclonal antibody (Product #PA5-18598) at 5 µg/mL. The Human Cortex tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive data in Antibodypedia
