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- Antibody Data
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- References [14]
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- Other assay [1]
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- Product number
- NBP1-05198 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-05198, RRID:AB_1556315
- Product name
- Chicken Polyclonal GFAP Antibody
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Chicken/Avian
- Isotype
- IgY
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Ependymal Vps35 Promotes Ependymal Cell Differentiation and Survival, Suppresses Microglial Activation, and Prevents Neonatal Hydrocephalus.
Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits.
Maternal immune activation modifies the course of Niemann-pick disease, type C1 in a gender specific manner.
Effect of cerebral small vessel disease on cognitive function and TLR4 expression in hippocampus.
Pseudovirus rVSVΔG-ZEBOV-GP Infects Neurons in Retina and CNS, Causing Apoptosis and Neurodegeneration in Neonatal Mice.
Unique molecular signature in mucolipidosis type IV microglia.
Autophagy Mediates Astrogenesis in Adult Hippocampal Neural Stem Cells.
Required growth facilitators propel axon regeneration across complete spinal cord injury.
Delivery of immunoglobulin G antibodies to the rat nervous system following intranasal administration: Distribution, dose-response, and mechanisms of delivery.
A pathway for Parkinson's Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain.
The role of inflammation on the functionality of intracortical microelectrodes.
Necroptosis inhibition as a therapy for Niemann-Pick disease, type C1: Inhibition of RIP kinases and combination therapy with 2-hydroxypropyl-β-cyclodextrin.
Delayed injury of hippocampal interneurons after neonatal hypoxia-ischemia and therapeutic hypothermia in a murine model.
Pulmonary surfactant protein a is expressed in mouse retina by Müller cells and impacts neovascularization in oxygen-induced retinopathy.
Wu KY, Tang FL, Lee D, Zhao Y, Song H, Zhu XJ, Mei L, Xiong WC
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 May 6;40(19):3862-3879
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 May 6;40(19):3862-3879
Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits.
Jung S, Choe S, Woo H, Jeong H, An HK, Moon H, Ryu HY, Yeo BK, Lee YW, Choi H, Mun JY, Sun W, Choe HK, Kim EK, Yu SW
Autophagy 2020 Mar;16(3):512-530
Autophagy 2020 Mar;16(3):512-530
Maternal immune activation modifies the course of Niemann-pick disease, type C1 in a gender specific manner.
Cougnoux A, Fellmeth M, Gu T, Davidson CD, Gibson AL, Pavan WJ, Porter FD
Molecular genetics and metabolism 2020 Feb;129(2):165-170
Molecular genetics and metabolism 2020 Feb;129(2):165-170
Effect of cerebral small vessel disease on cognitive function and TLR4 expression in hippocampus.
Zhang Y, Zhang Y, Wu R, Gao F, Zang P, Hu X, Gu C
Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia 2019 Sep;67:210-214
Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia 2019 Sep;67:210-214
Pseudovirus rVSVΔG-ZEBOV-GP Infects Neurons in Retina and CNS, Causing Apoptosis and Neurodegeneration in Neonatal Mice.
McWilliams IL, Kielczewski JL, Ireland DDC, Sykes JS, Lewkowicz AP, Konduru K, Xu BC, Chan CC, Caspi RR, Manangeeswaran M, Verthelyi D
Cell reports 2019 Feb 12;26(7):1718-1726.e4
Cell reports 2019 Feb 12;26(7):1718-1726.e4
Unique molecular signature in mucolipidosis type IV microglia.
Cougnoux A, Drummond RA, Fellmeth M, Navid F, Collar AL, Iben J, Kulkarni AB, Pickel J, Schiffmann R, Wassif CA, Cawley NX, Lionakis MS, Porter FD
Journal of neuroinflammation 2019 Dec 28;16(1):276
Journal of neuroinflammation 2019 Dec 28;16(1):276
Autophagy Mediates Astrogenesis in Adult Hippocampal Neural Stem Cells.
Ha S, Jeong SH, Yi K, Chu JJ, Kim S, Kim EK, Yu SW
Experimental neurobiology 2019 Apr;28(2):229-246
Experimental neurobiology 2019 Apr;28(2):229-246
Required growth facilitators propel axon regeneration across complete spinal cord injury.
Anderson MA, O'Shea TM, Burda JE, Ao Y, Barlatey SL, Bernstein AM, Kim JH, James ND, Rogers A, Kato B, Wollenberg AL, Kawaguchi R, Coppola G, Wang C, Deming TJ, He Z, Courtine G, Sofroniew MV
Nature 2018 Sep;561(7723):396-400
Nature 2018 Sep;561(7723):396-400
Delivery of immunoglobulin G antibodies to the rat nervous system following intranasal administration: Distribution, dose-response, and mechanisms of delivery.
Kumar NN, Lochhead JJ, Pizzo ME, Nehra G, Boroumand S, Greene G, Thorne RG
Journal of controlled release : official journal of the Controlled Release Society 2018 Sep 28;286:467-484
Journal of controlled release : official journal of the Controlled Release Society 2018 Sep 28;286:467-484
A pathway for Parkinson's Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain.
Dhekne HS, Yanatori I, Gomez RC, Tonelli F, Diez F, Schüle B, Steger M, Alessi DR, Pfeffer SR
eLife 2018 Nov 6;7
eLife 2018 Nov 6;7
The role of inflammation on the functionality of intracortical microelectrodes.
Gaire J, Lee HC, Hilborn N, Ward R, Regan M, Otto KJ
Journal of neural engineering 2018 Dec;15(6):066027
Journal of neural engineering 2018 Dec;15(6):066027
Necroptosis inhibition as a therapy for Niemann-Pick disease, type C1: Inhibition of RIP kinases and combination therapy with 2-hydroxypropyl-β-cyclodextrin.
Cougnoux A, Clifford S, Salman A, Ng SL, Bertin J, Porter FD
Molecular genetics and metabolism 2018 Dec;125(4):345-350
Molecular genetics and metabolism 2018 Dec;125(4):345-350
Delayed injury of hippocampal interneurons after neonatal hypoxia-ischemia and therapeutic hypothermia in a murine model.
Chavez-Valdez R, Emerson P, Goffigan-Holmes J, Kirkwood A, Martin LJ, Northington FJ
Hippocampus 2018 Aug;28(8):617-630
Hippocampus 2018 Aug;28(8):617-630
Pulmonary surfactant protein a is expressed in mouse retina by Müller cells and impacts neovascularization in oxygen-induced retinopathy.
Bhatti F, Ball G, Hobbs R, Linens A, Munzar S, Akram R, Barber AJ, Anderson M, Elliott M, Edwards M
Investigative ophthalmology & visual science 2014 Nov 18;56(1):232-42
Investigative ophthalmology & visual science 2014 Nov 18;56(1):232-42
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GFAP Antibody [NBP1-05198] - Analysis of whole brain lysates using chicken pAb to GFAP, NBP1-05198, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain. The strong band at about 50 kDa corresponds to the GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry Free-Floating: GFAP Antibody [NBP1-05198] - Staining of a section of mouse hippocampus stained with chicken pAb to GFAP, NBP1-05198, dilution 1:5,000 in green and costained with rabbit pAb to FOX3/NeuN, dilution 1:5,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, mouse brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The GFAP antibody stains a network of astroglial cells while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons.
