Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-47844 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UGT1A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-human Glucuronosyltransferase 1A1/UGT1A1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47844) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Glucuronosyltransferase 1A1/UGT1A1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UGT1A1 in human liver tissue. Samples were incubated in UGT1A1 polyclonal antibody (Product # PA5-47844) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Glucuronosyltransferase 1A1/UGT1A1 at approximately 57 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of UGT1A1 in immersion fixed HepG2 human hepatocellular carcinoma cell line. Samples were incubated in UGT1A1 polyclonal antibody (Product # PA5-47844) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of UGT1A1 in immersion fixed paraffin-embedded sections of human liver. Samples were incubated in UGT1A1 polyclonal antibody (Product # PA5-47844) using a dilution of 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent‚Basic. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes.