Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- A01865-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-UGT1A1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Glucuronosyltransferase 1A1/UGT1A1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. Glucuronosyltransferase 1A1/UGT1A1 information: Subcellular Localization: Isoform 1: Microsome; Tissue Specificity: Isoform 1 and isoform 2 are expressed in liver, colon and small intestine. Isoform 2 but not isoform 1 is expressed in kidney. Isoform 1 and isoform 2 are not expressed in esophagus. Not expressed in skin.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references CRISPR-Cas9-mediated somatic correction of a one-base deletion in the Ugt1a gene ameliorates hyperbilirubinemia in Crigler-Najjar syndrome mice.
Activation of Alternative Bilirubin Clearance Pathways Partially Reduces Hyperbilirubinemia in a Mouse Model Lacking Functional Ugt1a1 Activity.
Bortolussi G, Iaconcig A, Canarutto G, Porro F, Ferrucci F, Galletta C, Díaz-Muñoz C, Rawat V, De Caneva A, Olajide OJ, Zentilin L, Piazza S, Bočkor L, Muro AF
Molecular therapy. Methods & clinical development 2023 Dec 14;31:101161
Molecular therapy. Methods & clinical development 2023 Dec 14;31:101161
Activation of Alternative Bilirubin Clearance Pathways Partially Reduces Hyperbilirubinemia in a Mouse Model Lacking Functional Ugt1a1 Activity.
Banerjee B, Olajide OJ, Bortolussi G, Muro AF
International journal of molecular sciences 2022 Sep 14;23(18)
International journal of molecular sciences 2022 Sep 14;23(18)
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of UGT1A1 using anti-UGT1A1 antibody (A01865-1). UGT1A1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UGT1A1 Antibody (A01865-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image