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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00010687-M10 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010687-M10, RRID:AB_1136990
- Product name
- PNMA2 monoclonal antibody (M10), clone 4F10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant PNMA2.
- Antigen sequence
MALALLEDWCRIMSVDEQKSLMVTGIPADFEEAEI
QEVLQETLKSLGRYRLLGKIFRKQENANAVLLELL
EDTDVSAIPSEVQGKGGVWKVIFKTPNQDTEFLER
LNLFLEKEGQTVSGMFRALGQEGVSPATVPCISPE
LLAHLLGQAMAHAPQPLLPMRYRKLRVFSGSAVPA
PEEESFEVWLEQATEIVKEWPVTEAEKKRWLAESL
RGPALDLMHIVQADNPSISVEECLEAFKQVFGSLE
SRRTAQVRYLKTYQEEGEKVSAYVLRLETLLRRAV
EKRAIPRRIADQVRLEQVMAGATLNQMLWCRLREL
KDQGPPPSFLELMKVIREEEEEEASFENESIEEPE
ERDGYGRWNHEGDD- Isotype
- IgG
- Antibody clone number
- 4F10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PNMA2 expression in transfected 293T cell line by PNMA2 monoclonal antibody (M10), clone 4F10.Lane 1: PNMA2 transfected lysate (Predicted MW: 41.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PNMA2 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PNMA2 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PNMA2 transfected lysate using anti-PNMA2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PNMA2 monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
