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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-30982 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, mouse brain, rat brain.
- Concentration
- 0.75 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MA2 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a MA2 Polyclonal Antibody (Product # PA5-30982) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MA2 Polyclonal Antibody (Product # PA5-30982). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. MA2 Polyclonal Antibody (Product # PA5-30982) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MA2 Polyclonal Antibody (Product # PA5-30982). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 10% SDS PAGE. MA2 Polyclonal Antibody (Product # PA5-30982) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MA2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: MA2 Polyclonal Antibody (Product # PA5-30982) diluted at 1:500. Red: phalloidin, a cytoskeleton marker.
