PA5-120366

antibody from Invitrogen Antibodies

Targeting: PLEKHB2 EVT2, FLJ20783

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120366

Provider

Invitrogen Antibodies

Product name

PLEKHB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: HT-29, A-549, BxPC-3, A-431. The target is usually found in the following locations: Peripheral membrane protein, Recycling endosome membrane.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.51 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PLEKHB2 in various cell lines using a PLEKHB2 Polyclonal antibody (Product # PA5-120366) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 30s.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PLEKHB2 in human liver cancer using a Polyclonal antibody (Product # PA5-120366) at a dilution of 1:100.