AF174

antibody from R&D Systems

Targeting: TNFRSF13B CD267, IGAD2, TACI

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF174

Provider

R&D Systems

Product name

Human TACI/TNFRSF13B Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human TACI in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TACI and recombinant human BCMA is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q53F36


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

IFN-γ stimulates CpG-induced IL-10 production in B cells via p38 and JNK signalling pathways.

Imbrechts M, De Samblancx K, Fierens K, Brisse E, Vandenhaute J, Mitera T, Libert C, Smets I, Goris A, Wouters C, Matthys P
European journal of immunology 2018 Sep;48(9):1506-1521

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The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.

Saulep-Easton D, Vincent FB, Quah PS, Wei A, Ting SB, Croce CM, Tam C, Mackay F
Leukemia 2016 Jan;30(1):163-72

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The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity.

Hoffmann FS, Kuhn PH, Laurent SA, Hauck SM, Berer K, Wendlinger SA, Krumbholz M, Khademi M, Olsson T, Dreyling M, Pfister HW, Alexander T, Hiepe F, Kümpfel T, Crawford HC, Wekerle H, Hohlfeld R, Lichtenthaler SF, Meinl E
Journal of immunology (Baltimore, Md. : 1950) 2015 Jan 15;194(2):542-52

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TACI mutations and impaired B-cell function in subjects with CVID and healthy heterozygotes.

Martinez-Gallo M, Radigan L, Almejún MB, Martínez-Pomar N, Matamoros N, Cunningham-Rundles C
The Journal of allergy and clinical immunology 2013 Feb;131(2):468-76

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The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88.

He B, Santamaria R, Xu W, Cols M, Chen K, Puga I, Shan M, Xiong H, Bussel JB, Chiu A, Puel A, Reichenbach J, Marodi L, Döffinger R, Vasconcelos J, Issekutz A, Krause J, Davies G, Li X, Grimbacher B, Plebani A, Meffre E, Picard C, Cunningham-Rundles C, Casanova JL, Cerutti A
Nature immunology 2010 Sep;11(9):836-45

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Identification of transitional type II B cells in the salivary glands of patients with Sjögren's syndrome.

Daridon C, Pers JO, Devauchelle V, Martins-Carvalho C, Hutin P, Pennec YL, Saraux A, Youinou P
Arthritis and rheumatism 2006 Jul;54(7):2280-8

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

TACI/TNFRSF13B in Human Spleen. TACI/TNFRSF13B was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human TACI/TNFRSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF174) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific labeling was localized to lymphocytes in the marginal zone. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.