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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-30048 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Actin Alpha 1 Skeletal Muscle Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 0.7 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Actin Alpha 1 Skeletal Muscle Antibody [NBP2-30048] - ACTA1/a-actin Antibody (C-term) (NBP2-30048) western blot analysis in A549,RD cell line and human placenta lysates (35ug/lane).This demonstrates the ACTA1/a-actin antibody detected the ACTA1/a-actin protein (arrow).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Skeletal Muscle Antibody [NBP2-30048] - Confocal immunofluorescent analysis of ACTA1/alpha-actin Antibody (C-term)(NBP2-30048) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Actin Alpha 1 Skeletal Muscle Antibody [NBP2-30048] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1/alpha-actin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Actin Alpha 1 Skeletal Muscle Antibody [NBP2-30048] - Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
