AM1965a

antibody from Abgent

Targeting: ACTA1 ACTA, NEM3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM1965a

Provider

Abgent

Proper citation

Abgent Cat#AM1965a, RRID:AB_11138233

Product name

ACTA1 Antibody (Ascites)

Antibody type

Monoclonal

Antigen

Recombinant Protein

Description

Mouse Monoclonal Antibody (Mab)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ACTA1 Antibody (Cat. #AM1965a) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ACTA1 antibody detected the ACTA1 protein (arrow).

Primary Ab dilution

1:1000~16000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of ACTA1 Antibody (Ascites)(Cat#AM1965a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ACTA1 Antibody (Ascites) (Cat. #AM1965a) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50