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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- AF4000 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Actin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat Actin in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fatty acid-binding protein 5 (FABP5) regulates cognitive function both by decreasing anandamide levels and by activating the nuclear receptor peroxisome proliferator-activated receptor β/δ (PPARβ/δ) in the brain.
Nuclear trafficking of the HIV-1 pre-integration complex depends on the ADAM10 intracellular domain.
Microbial exposure early in life regulates airway inflammation in mice after infection with Streptococcus pneumoniae with enhancement of local resistance.
cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
Yu S, Levi L, Casadesus G, Kunos G, Noy N
The Journal of biological chemistry 2014 May 2;289(18):12748-58
The Journal of biological chemistry 2014 May 2;289(18):12748-58
Nuclear trafficking of the HIV-1 pre-integration complex depends on the ADAM10 intracellular domain.
Endsley MA, Somasunderam AD, Li G, Oezguen N, Thiviyanathan V, Murray JL, Rubin DH, Hodge TW, O'Brien WA, Lewis B, Ferguson MR
Virology 2014 Apr;454-455:60-6
Virology 2014 Apr;454-455:60-6
Microbial exposure early in life regulates airway inflammation in mice after infection with Streptococcus pneumoniae with enhancement of local resistance.
Yasuda Y, Matsumura Y, Kasahara K, Ouji N, Sugiura S, Mikasa K, Kita E
American journal of physiology. Lung cellular and molecular physiology 2010 Jan;298(1):L67-78
American journal of physiology. Lung cellular and molecular physiology 2010 Jan;298(1):L67-78
cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
Siddappa R, Martens A, Doorn J, Leusink A, Olivo C, Licht R, van Rijn L, Gaspar C, Fodde R, Janssen F, van Blitterswijk C, de Boer J
Proceedings of the National Academy of Sciences of the United States of America 2008 May 20;105(20):7281-6
Proceedings of the National Academy of Sciences of the United States of America 2008 May 20;105(20):7281-6
Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
Biotechnology and bioengineering 2007 Aug 15;97(6):1617-25
Biotechnology and bioengineering 2007 Aug 15;97(6):1617-25
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat Actin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Actin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Actin in HeLa Human Cell Line. Actin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Actin in HEK293 Human Cell Line. Actin was detected in immersion fixed HEK293 human embryonic kidney cell line using Sheep Anti-Human/Mouse/Rat Actin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4000) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
