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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C413476 - Provider product page
- Provider
- LSBio
- Product name
- ACTA1 / Skeletal Muscle Actin Antibody LS-C413476
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Storage
- Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of Alpha-actin-1 expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of Alpha-actin-1 from 0.5mg mouse brain whole cell extract lysate; using Anti-Alpha-actin-1 Antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of Alpha-actin-1 from 0.5mg mouse brain whole cell extract lysate; using Anti-Alpha-actin-1 Antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemical analysis of Alpha-actin-1 staining in mouse skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
