66019-1-IG

antibody from Invitrogen Antibodies

Targeting: AHCY SAHH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

66019-1-IG

Provider

Invitrogen Antibodies

Product name

AHCY Monoclonal Antibody (1F9B10)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: QTLYFKDGP LNMILDDGGD LTNLIHTKYP QLLPGIRGIS EETTTGVHNL YKMMANGILK VPAINVNDSV TKSKFDNLYG CRESLIDGIK RATDVMIAGK VAVVAGYGDV GKGCAQALRG FGARVIITEI DPINALQAAM EGYEVTTMDE ACQEGNIFVT TTGCIDIILG RHFEQMKDDA IVCNIGHFDV EIDVKWLNEN AVEKVNIKPQ VDRYRLKNGR RIILLAEGRL VNLGCAMGHP SFVMSNSFTN QVMAQIELWT HPDKYPVGVH FLPKKLDEAV AEAHLGKLNV KLTKLTEKQA QYLGMSCDGP FKPDHYRY (116-432 aa encoded by BC011606)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1F9B10

Vial size

150 µL

Concentration

1.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human testis tissue were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-IG (AHCY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using AHCY antibody 66019-1-IG at 1:25 dilution and Rhodamine-labeled goat anti-mouse IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 66019-1-IG (AHCY antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 66019-1-Ig (AHCY antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 66019-1-Ig (AHCY antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 66019-1-IG (AHCY antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 66019-1-IG (AHCY antibody) at dilution of 1:50 (under 40x lens).