Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF6466 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Adenosylhomocysteinase/AHCY Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Adenosylhomocysteinase/AHCY in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P23526
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Inhibition of cellular methyltransferases promotes endothelial cell activation by suppressing glutathione peroxidase 1 protein expression.
Barroso M, Florindo C, Kalwa H, Silva Z, Turanov AA, Carlson BA, de Almeida IT, Blom HJ, Gladyshev VN, Hatfield DL, Michel T, Castro R, Loscalzo J, Handy DE
The Journal of biological chemistry 2014 May 30;289(22):15350-62
The Journal of biological chemistry 2014 May 30;289(22):15350-62
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Adenosylhomocysteinase/AHCY by Western Blot. Western blot shows lysates of Bowes human melanoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Adenosylhomocysteinase/AHCY Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6466) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adenosylhomocysteinase/AHCY at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.