Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R20178 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Human Kappa Light Chain Antibody
- Antibody type
- Recombinant
- Description
- Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM126
- Vial size
- 100 µg
- Concentration
- 1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant Human Kappa Light Chain antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA of human immunoglobulins shows the recombinant Human Kappa Light Chain antibody reacts to the kappa light chain of hIgs. No cross reactivity with the lambda light chain, mouse/rat/goat IgG.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA Titration: the plate was coated with different amounts of human IgG4κ. A serial dilution of the recombinant Human Kappa Light Chain antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with recombinant Human Kappa Light Chain antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.