Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R20178BTN - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Human Kappa Light Chain Antibody
- Antibody type
- Recombinant
- Description
- Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Antibody clone number
- RM126
- Vial size
- 50 µg
- Concentration
- 1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant Human Kappa Light Chain antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA of human immunoglobulins shows the recombinant Human Kappa Light Chain antibody reacts to the kappa light chain of human immunoglobulins. No cross reactivity with the lambda light chain, mouse IgG, rat IgG, or goat IgG.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA Titration: the plate was coated with different amounts of human IgG4κ. A serial dilution of the recombinant Human Kappa Light Chain antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with recombinant Human Kappa Light Chain antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.