Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-5139-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD85k (ILT3) Monoclonal Antibody (ZM4.1), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody ZM4.1 recognizes ILT3, also known as CD85k. ILT3 is a member of the large family of ILT molecules (Immunologulin-like Transcripts, Leucocyte Ig-like Receptors (LIRs) and Monocyte/Macrophage Ig-like Receptors (MIRs). ILT3 is a 60kDa transmembrane protein with two Ig domains and ITIMs. Expression is found on monocytes, macrophages, and dendritic cells. High levels of ILT3 expression is also found on plasmacytoid dendritic cells (pDCs). ILT3 expression by DCs has been shown to be required to induce Regulatory Tcells (tregs). Addition of ZM4.1, like ZM3.8, blocks the suppressive function of the Tregs in in vitro culture systems with allogenic DC's. Applications Reported: This ZM4.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This ZM4.1 antibody has been pre-titrated and tested flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- ZM4.1
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.
NOD1 modulates IL-10 signalling in human dendritic cells.
Interferon beta and vitamin D synergize to induce immunoregulatory receptors on peripheral blood monocytes of multiple sclerosis patients.
Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells.
A novel inhibitory receptor (ILT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen processing.
Tomić S, Ilić N, Kokol V, Gruden-Movsesijan A, Mihajlović D, Bekić M, Sofronić-Milosavljević L, Čolić M, Vučević D
International journal of nanomedicine 2018;13:6941-6960
International journal of nanomedicine 2018;13:6941-6960
NOD1 modulates IL-10 signalling in human dendritic cells.
Neuper T, Ellwanger K, Schwarz H, Kufer TA, Duschl A, Horejs-Hoeck J
Scientific reports 2017 Apr 21;7(1):1005
Scientific reports 2017 Apr 21;7(1):1005
Interferon beta and vitamin D synergize to induce immunoregulatory receptors on peripheral blood monocytes of multiple sclerosis patients.
Waschbisch A, Sanderson N, Krumbholz M, Vlad G, Theil D, Schwab S, Mäurer M, Derfuss T
PloS one 2014;9(12):e115488
PloS one 2014;9(12):e115488
Mesenchymal stem cells from periapical lesions modulate differentiation and functional properties of monocyte-derived dendritic cells.
Dokić J, Tomić S, Marković M, Milosavljević P, Colić M
European journal of immunology 2013 Jul;43(7):1862-72
European journal of immunology 2013 Jul;43(7):1862-72
A novel inhibitory receptor (ILT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen processing.
Cella M, Döhring C, Samaridis J, Dessing M, Brockhaus M, Lanzavecchia A, Colonna M
The Journal of experimental medicine 1997 May 19;185(10):1743-51
The Journal of experimental medicine 1997 May 19;185(10):1743-51
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD123 FITC (Product # 11-1239-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD85k (ILT3) PE (right). Cells in the monocyte gate were used for analysis.
- Conjugate
- Yellow dye