Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-83480 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83480, RRID:AB_11040394
- Product name
- Rabbit Polyclonal GPRASP2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human GPRASP2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GPRASP2 Antibody [NBP1-83480] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-248
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GPRASP2 Antibody [NBP1-83480] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-GPRASP2 antibody. Remaining relative intensity is presented.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GPRASP2 Antibody [NBP1-83480] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GPRASP2 Antibody [NBP1-83480] - Staining of human cerebral cortex shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GPRASP2 Antibody [NBP1-83480] - Staining of human duodenum shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GPRASP2 Antibody [NBP1-83480] - Staining in human cerebral cortex and duodenum tissues using anti-GPRASP2 antibody. Corresponding GPRASP2 RNA-seq data are presented for the same tissues.