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antibody from NSJ Bioreagents
Targeting: CD68
DKFZp686M18236, GP110, LAMP4, macrosialin, SCARD1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- V2074 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD68 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific CD68 antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Immunohistochemistry applications with human, mouse and rat, cat samples.
- Reactivity
- Human, Mouse, Rat, Feline
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- C68/684
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human spleen lysate with CD68 antibody (clone C68/684). Expected molecular weight: 37-110 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed human U-87 MG cells with CD68 antibody (clone C68/684, green) and Reddot nuclear stain (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human tonsil (10X) stained with CD68 antibody (C68/684).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed human U-87 MG cells with CD63 antibody (clone C68/684); Red=isotype control, Blue= CD63 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free CD68 antibody (clone C68/684) as confirmation of integrity and purity.
