NBP2-32831-0.1mg
antibody from Novus Biologicals
Targeting: CD68
DKFZp686M18236, GP110, LAMP4, macrosialin, SCARD1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [3]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-32831-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD68/SR-D1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Feline, Porcine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (SPM130) [NBP2-32831] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD68 MAb (SPM130).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (SPM130) [NBP2-32831] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD68 MAb (SPM130).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD68/SR-D1 Antibody (SPM130) [NBP2-32831] - The antibody was incubated with feline spleen section at 1:100 and the slide was further stained with Alexa secondary antibody. Image was captured with epifluorescence microscope. This image was submitted via customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD68/SR-D1 Antibody (SPM130) [NBP2-32831] - Using the Alexa Fluor 647 direct conjugate Intracellular staining of CD14+ hPBMCs with CD68/SR-D1 (SPM130) NBP234736AF647 and a matched isotype control NBP2-27287AF647. Cells were fixed with 4% PFA and permebilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/ml for 30 minutes at room temperature. A co-stain was performed with NB100-77758AF488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD68/SR-D1 Antibody (SPM130) [NBP2-32831] - Flow Cytometry: CD68/SR-D1 Antibody (SPM130) [Biotin] [NBP2-34736B] - Mouse peripheral blood cells were unstained (left) or stained (right) with CD68/SR-D1 antibody and anti-biotin alexa-fluor 488 secondary antibody. Image from verified customer review.