Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C153796 - Provider product page
- Provider
- LSBio
- Product name
- LYZ / Lysozyme Antibody LS-C153796
- Antibody type
- Polyclonal
- Description
- Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
- Reactivity
- Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
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- Experimental details
- Western blot of Rabbit anti-Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10000 for 1.5 hrs at RT. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5000 with Dylight 549 conjugated secondary antibody at 1:10000 for 1.5 hrs at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.