LS-C209251

antibody from LSBio

Targeting: LYZ

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C209251

Provider

LSBio

Product name

LYZ / Lysozyme Antibody (Lyophilized, HRP) LS-C209251

Antibody type

Polyclonal

Description

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Reactivity

Chicken/Avian

Host

Rabbit

Conjugate

Horseradish Peroxidase

Isotype

IgG

Storage

Store vial at 4°C prior to opening. Restore with 0.1 ml of deionized water or equivalent. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot of Rabbit anti-Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4 degrees C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4 degrees C. Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.