LS-C195847

antibody from LSBio

Targeting: MPO

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C195847

Provider

LSBio

Product name

MPO / Myeloperoxidase Antibody LS-C195847

Antibody type

Polyclonal

Description

Purified

Reactivity

Human

Host

Rabbit

Storage

Prior to reconstitution, 4°C. Following reconstitution store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot of Myeloperoxidase antibody. Myeloperoxidase antibody was used at a 1:5000 dilution to detect 1. 0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye 800 conjugated Goat anti Rabbit IgG (H + L) for 30 min at room temperature. LICOR''s Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.