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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- MA1-34067 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Myeloperoxidase Monoclonal Antibody (2C7)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- A suggested positive control for this product is bone marrow.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2C7
- Vial size
- 40 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1α, IL-1β, IL-12b, and CCL4 from differentiated HL-60 cells.
Naegelen I, Plançon S, Nicot N, Kaoma T, Muller A, Vallar L, Tschirhart EJ, Bréchard S
Journal of leukocyte biology 2015 Mar;97(3):557-71
Journal of leukocyte biology 2015 Mar;97(3):557-71
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MPO was performed using HL-60 cells treated with 1 micromolar Retinoic Acid for 96Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Myeloperoxidase Monoclonal Antibody (2C7) (Product # MA1-34067) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at 1:2000 dilution for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HL-60 treated with Retinoic acid showing reduced signal of MPO in cytoplasmic vesicle like structures. Panel e represents untreated HL-60 cells with intense signal in cytoplasmic vesicle like structures for MPO. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Myeloperoxidase in human bone marrow tissue using Myeloperoxidase Monoclonal Antibody (2C7) (Product # MA1-34067).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of LAIR1 was performed in human peripheral blood granulocytes using LAIR1 Monoclonal Antibody (NKTA255) (Product # MA1-33625).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of LAIR1 was performed in human peripheral blood granulocytes using LAIR1 Monoclonal Antibody (NKTA255) (Product # MA1-33625).
