AF3667

antibody from Novus Biologicals

Targeting: MPO

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3667

Provider

Novus Biologicals

Product name

Goat Polyclonal Myeloperoxidase/MPO Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human and mouse Myeloperoxidase/MPO in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse EPPO is observed.

Reactivity

Human, Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Locally instructed CXCR4^hi neutrophils trigger environment-driven allergic asthma through the release of neutrophil extracellular traps.

Radermecker C, Sabatel C, Vanwinge C, Ruscitti C, Maréchal P, Perin F, Schyns J, Rocks N, Toussaint M, Cataldo D, Johnston SL, Bureau F, Marichal T
Nature immunology 2019 Nov;20(11):1444-1455

---

Acute Liver Injury after CCl₄ Administration is Independent of Smad7 Expression in Myeloid Cells.

Endig J, Unrau L, Sprezyna P, Rading S, Karsak M, Goltz D, Heukamp LC, Tiegs G, Diehl L
International journal of molecular sciences 2019 Nov 6;20(22)

---

Keratinocyte-derived IκBζ drives psoriasis and associated systemic inflammation.

Lorscheid S, Müller A, Löffler J, Resch C, Bucher P, Kurschus FC, Waisman A, Schäkel K, Hailfinger S, Schulze-Osthoff K, Kramer D
JCI insight 2019 Nov 14;4(22)

---

Qingnao dripping pills mediate immune-inflammatory response and MAPK signaling pathway after acute ischemic stroke in rats.

Zeng Z, Zhang Y, Liang X, Wang F, Zhao J, Xu Z, Liu X, Liu X
Journal of pharmacological sciences 2019 Mar;139(3):143-150

---

Tofacitinib enhances delivery of antibody-based therapeutics to tumor cells through modulation of inflammatory cells.

Simon N, Antignani A, Hewitt SM, Gadina M, Alewine C, FitzGerald D
JCI insight 2019 Mar 7;4(5)

---

Neutrophils escort circulating tumour cells to enable cell cycle progression.

Szczerba BM, Castro-Giner F, Vetter M, Krol I, Gkountela S, Landin J, Scheidmann MC, Donato C, Scherrer R, Singer J, Beisel C, Kurzeder C, Heinzelmann-Schwarz V, Rochlitz C, Weber WP, Beerenwinkel N, Aceto N
Nature 2019 Feb;566(7745):553-557

---

GWAS for Interleukin-1β levels in gingival crevicular fluid identifies IL37 variants in periodontal inflammation.

Offenbacher S, Jiao Y, Kim SJ, Marchesan J, Moss KL, Jing L, Divaris K, Bencharit S, Agler CS, Morelli T, Zhang S, Sun L, Seaman WT, Cowley D, Barros SP, Beck JD, Munz M, Schaefer AS, North KE
Nature communications 2018 Sep 11;9(1):3686

---

Mesenchymal stem cells attenuate blood-brain barrier leakage after cerebral ischemia in mice.

Cheng Z, Wang L, Qu M, Liang H, Li W, Li Y, Deng L, Zhang Z, Yang GY
Journal of neuroinflammation 2018 May 3;15(1):135

---

Reg3β is associated with cardiac inflammation and provides prognostic information in patients with acute coronary syndrome.

Lörchner H, Widera C, Hou Y, Elsässer A, Warnecke H, Giannitsis E, Hulot JS, Braun T, Wollert KC, Pöling J
International journal of cardiology 2018 May 1;258:7-13

---

Matrix metalloproteinase-9 deficiency protects mice from severe influenza A viral infection.

Rojas-Quintero J, Wang X, Tipper J, Burkett PR, Zuñiga J, Ashtekar AR, Polverino F, Rout A, Yambayev I, Hernández C, Jimenez L, Ramírez G, Harrod KS, Owen CA
JCI insight 2018 Dec 20;3(24)

---

Cell-penetrating interactomic inhibition of nuclear factor-kappa B in a mouse model of postoperative cognitive dysfunction.

Cheon SY, Kim JM, Kam EH, Ho CC, Kim EJ, Chung S, Jeong JH, Lee DD, Lee SW, Koo BN
Scientific reports 2017 Oct 18;7(1):13482

---

Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation.

Toussaint M, Jackson DJ, Swieboda D, Guedán A, Tsourouktsoglou TD, Ching YM, Radermecker C, Makrinioti H, Aniscenko J, Bartlett NW, Edwards MR, Solari R, Farnir F, Papayannopoulos V, Bureau F, Marichal T, Johnston SL
Nature medicine 2017 Jun;23(6):681-691

---

Topical Fibronectin Improves Wound Healing of Irradiated Skin.

Johnson MB, Pang B, Gardner DJ, Niknam-Benia S, Soundarajan V, Bramos A, Perrault DP, Banks K, Lee GK, Baker RY, Kim GH, Lee S, Chai Y, Chen M, Li W, Kwong L, Hong YK, Wong AK
Scientific reports 2017 Jun 20;7(1):3876

---

Obesity promotes prolonged ovalbumin-induced airway inflammation modulating T helper type 1 (Th1), Th2 and Th17 immune responses in BALB/c mice.

Silva FMC, Oliveira EE, Gouveia ACC, Brugiolo ASS, Alves CC, Correa JOA, Gameiro J, Mattes J, Teixeira HC, Ferreira AP
Clinical and experimental immunology 2017 Jul;189(1):47-59

---

The blood-borne sialyltransferase ST6Gal-1 is a negative systemic regulator of granulopoiesis.

Dougher CWL, Buffone A Jr, Nemeth MJ, Nasirikenari M, Irons EE, Bogner PN, Lau JTY
Journal of leukocyte biology 2017 Aug;102(2):507-516

---

Beneficial Effects of Sodium Phenylbutyrate Administration during Infection with Salmonella enterica Serovar Typhimurium.

Jellbauer S, Perez Lopez A, Behnsen J, Gao N, Nguyen T, Murphy C, Edwards RA, Raffatellu M
Infection and immunity 2016 Sep;84(9):2639-52

---

Swimming Motility Mediates the Formation of Neutrophil Extracellular Traps Induced by Flagellated Pseudomonas aeruginosa.

Floyd M, Winn M, Cullen C, Sil P, Chassaing B, Yoo DG, Gewirtz AT, Goldberg JB, McCarter LL, Rada B
PLoS pathogens 2016 Nov;12(11):e1005987

---

Neutrophil Attack Triggers Extracellular Trap-Dependent Candida Cell Wall Remodeling and Altered Immune Recognition.

Hopke A, Nicke N, Hidu EE, Degani G, Popolo L, Wheeler RT
PLoS pathogens 2016 May;12(5):e1005644

---

PAD4 Deficiency Decreases Inflammation and Susceptibility to Pregnancy Loss in a Mouse Model.

Erpenbeck L, Chowdhury CS, Zsengellér ZK, Gallant M, Burke SD, Cifuni S, Hahn S, Wagner DD, Karumanchi SA
Biology of reproduction 2016 Dec;95(6):132

---

Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking β-glucans.

PLOS ONE Staff.
PloS one 2015;10(3):e0119244

---

Rhadinovirus host entry by co-operative infection.

Lawler C, Milho R, May JS, Stevenson PG
PLoS pathogens 2015 Mar;11(3):e1004761

---

Lactobacillus rhamnosus GG and Bifidobacterium longum attenuate lung injury and inflammatory response in experimental sepsis.

Khailova L, Petrie B, Baird CH, Dominguez Rieg JA, Wischmeyer PE
PloS one 2014;9(5):e97861

---

IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.

Guabiraba R, Besnard AG, Menezes GB, Secher T, Jabir MS, Amaral SS, Braun H, Lima-Junior RC, Ribeiro RA, Cunha FQ, Teixeira MM, Beyaert R, Graham GJ, Liew FY
Mucosal immunology 2014 Sep;7(5):1079-93

---

The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.

Behnsen J, Jellbauer S, Wong CP, Edwards RA, George MD, Ouyang W, Raffatellu M
Immunity 2014 Feb 20;40(2):262-73

---

Transcriptomic analysis of host immune and cell death responses associated with the influenza A virus PB1-F2 protein.

Le Goffic R, Leymarie O, Chevalier C, Rebours E, Da Costa B, Vidic J, Descamps D, Sallenave JM, Rauch M, Samson M, Delmas B
PLoS pathogens 2011 Aug;7(8):e1002202

---

Tissue inhibitor of metalloproteinases 3 regulates resolution of inflammation following acute lung injury.

Gill SE, Huizar I, Bench EM, Sussman SW, Wang Y, Khokha R, Parks WC
The American journal of pathology 2010 Jan;176(1):64-73

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human/Mouse Myeloperoxidase/MPO by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, human neutrophil cells, and mouse spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Myeloperoxidase/MPO at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse Myeloperoxidase/MPO by Simple Western^TM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Myeloperoxidase/MPO in Human Spleen. Myeloperoxidase/MPO was detected in immersion fixed paraffin-embedded sections of human spleen using 3 µg/mL Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Myeloperoxidase/MPO in Mouse Spleen Tissue.