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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- R30233 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Myeloperoxidase Antibody MPO
- Antibody type
- Polyclonal
- Antigen
- An amino acid sequence from the C-terminus of the human protein (KNNIFMSNSYPRDFV) was used as the immunogen for this Myeloperoxidase antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- The lyophilized Myeloperoxidase antibody can be stored at 4°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Myeloperoxidase antibody and rat brain tissue lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HL60, 2) rat thymus, 3) mouse spleen and 4) mouse thymus tissue with Myeloperoxidase antibody. Expected molecular weight: 59-64 kDa (alpha chain, may be observed at higher molecular weights due to glycosylation), 150+ kDa (glycosylated mature form).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human spleen tissue with Myeloperoxidase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human appendicitis tissue with Myeloperoxidase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Myeloperoxidase antibody testing of human liver cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat lymph tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human tonsil tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse spleen tissue with Myeloperoxidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HL60 cells with Myeloperoxidase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Myeloperoxidase antibody.
