Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-1299-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Myeloperoxidase (MPO) Monoclonal Antibody (MPO455-8E6), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody MPO455-8E6 recognizes myeloperoxidase (MPO), a protein within the azurophilic granules of myeloid cells. MPO is a multimeric protein comprised of two 55 kDa subunits and two 15 kDa subunits. The larger subunits associate with a heme protein resulting in a greenish color. As an enzyme, MPO breaks down hydrogen peroxide and oxidizes tyrosine. The products of this reaction, hypochlorous acid and tyrosyl radical, cause the cytotoxic and killing effects characteristic of neutrophils. Myeloperoxidase is important in the diagnosis of some cancers and increases in serum levels have been shown to correlate with cardiac events. Applications Reported: This MPO455-8E6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MPO455-8E6 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeablization Buffer Set (cat. 88-8824). For best results, whole blood should first be stained with antibodies to surface antigens then treated with 1X RBC lysis buffer (cat. 00-4333) to lyse erythrocytes. Then, for intracellular staining for MPO, cells should be fixed with IC Fixation Buffer (cat. 00-8222) washed two times with Permeabilization Buffer (cat. 00-8333) and then incubated with MPO455-8E6 for 30-60 minutes. After washing, cells may be analyzed on a flow cytometer. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- MPO455-8E6
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Innate Response Activator (IRA) B Cells Reside in Human Tonsils and Internalize Bacteria In Vitro.
Chiappini N, Cantisani R, Pancotto L, Ruggiero P, Rosa D, Manetti A, Romano A, Montagnani F, Bertholet S, Castellino F, Del Giudice G
PloS one 2015;10(6):e0129879
PloS one 2015;10(6):e0129879
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human Myeloperoxidase (MPO) PE (right) using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Total viable cells were used for analysis.
- Conjugate
- Yellow dye