NB600-923-0.5ml

antibody from Novus Biologicals

Targeting: MPO

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB600-923-0.5ml

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Myeloperoxidase/MPO Antibody

Antibody type

Polyclonal

Description

Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Myeloperoxidase [Human White Cell].

Reactivity

Human

Host

Rabbit

Vial size

0.5 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Myeloperoxidase/MPO Antibody [NB600-923] - Detects multiple MPO subunits and chain combinations by western blot. Polyclonal rabbit-anti-Myeloperoxidase was used at a 1:5000 dilution to detect 1.0 ug of human myeloperoxidase. This antibody detects a multiple bands corresponding to 53 kDa and 15 kDa polypeptides and chain combinations forming 68 kDa and 106 kDa proteins. The staining of the 68 kDa band is so intense that is over saturates the signal detection. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 2 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 30 min at room temperature.