Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-64241 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-64241, RRID:AB_964676
- Product name
- Mouse Monoclonal Myeloperoxidase/MPO Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-64803 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. NB100-64803 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [FITC] [NB100-64241] - Staining of permeabilised human peripheral blood granulocytes with MOUSE ANTI HUMAN MYELOPEROXIDASE:FITC