NBP2-12481-0.1ml

antibody from Novus Biologicals

Targeting: MPO

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-12481-0.1ml

Provider

Novus Biologicals

Product name

Rabbit Monoclonal Myeloperoxidase/MPO Antibody

Antibody type

Monoclonal

Description

Protein A purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C. Do not freeze.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (SP72) [NBP2-12481] - 1/100 dilution, Human tonsil tissue by Immunohistochemistry.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Myeloperoxidase/MPO Antibody (SP72) [NBP2-12481] - HeLa cells stained with (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.