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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Other assay [1]
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- Product number
- DOM00001G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Myeloperoxidase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Excitation: 555 nm; Emission: 570 nm
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Submitted references Chronic ethanol feeding promotes azoxymethane and dextran sulfate sodium-induced colonic tumorigenesis potentially by enhancing mucosal inflammation.
Shukla PK, Chaudhry KK, Mir H, Gangwar R, Yadav N, Manda B, Meena AS, Rao R
BMC cancer 2016 Mar 7;16:189
BMC cancer 2016 Mar 7;16:189
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Myeloperoxidase Rabbit Polyclonal Antibody (Product # DOM00001G) and 87 kDa, 73 kDa, 60 kDa and 15 kDa bands corresponding to MPO was observed across cell lines except K-562 and A-431, also it was observed to decrease upon Retinoic Acid treatment. Membrane enriched extracts (30 µg lysate) of HL-60 (Lane 1), HL-60 treated with Retinoic Acid (1uM for 72 Hours) (Lane 2), K-562 (Lane 3) and A-431 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (15ug) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Myeloperoxidase using a polyclonal antibody (Product # DOM00001G).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Myeloperoxidase using a polyclonal antibody (Product # DOM00001G).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Myeloperoxidase using a polyclonal antibody (Product # DOM00001G).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Myeloperoxidase using a polyclonal antibody (Product # DOM00001G).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Ethanol elevates inflammatory cells in colonic mucosa of AOM/DSS-treated mice: AOM/DSS-induced colonic tumorigenesis was induced with (EF) or without (PF) 4 % ethanol feeding as described in Methods section. At the pre-cancerous stage (Day 44; 2 week after second course od DSS colitis) cryosections of distal colon were stained for myeloperoxidase (MPO) ( a ), Gr1 ( b ) and CD68 ( c ) and co-stained for F-actin and nucleus. Images presented are representative of n of 5 for each group
