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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- MA5-15480 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Myeloperoxidase Monoclonal Antibody (9B12G7,4D8B12,9B12D9,9C11A5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15480 targets Myeloperoxidase in indirect ELISA, WB applications and shows reactivity with Human samples. The MA5-15480 immunogen is purified recombinant fragment of MPO (aa1-193) expressed in E. Coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9B12G7,4D8B12,9B12D9,9C11A5
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Myeloperoxidase using a Myeloperoxidase monoclonal antibody (Product # MA5-15480) against a truncated Trx-MPO recombinant protein (1), truncated MBP-MPO (aa1-193) recombinant protein (2) and truncated MPO (aa165-745) human IgG Fc transfected CHO-K1 cell lysate (3).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Myeloperoxidase using a Myeloperoxidase monoclonal antibody (Product # MA5-15480) against a truncated Trx-MPO recombinant protein (1), truncated MBP-MPO (aa1-193) recombinant protein (2) and truncated MPO (aa165-745) human IgG Fc transfected CHO-K1 cell lysate (3).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Myeloperoxidase Monoclonal Antibody (Product # MA5-15480) and 87 kDa, 73 kDa and 60 kDa bands corresponding to MPO was observed across cell lines except K-562 and A-431, also it was observed to decrease upon Retinoic Acid treatment. Membrane enriched extracts (30 µg lysate) of HL-60 (Lane 1), HL-60 treated with Retinoic Acid (1uM for 72 Hours) (Lane 2), K-562 (Lane 3) and A-431 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
