MA5-15480

antibody from Invitrogen Antibodies

Targeting: MPO

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15480

Provider

Invitrogen Antibodies

Product name

Myeloperoxidase Monoclonal Antibody (9B12G7,4D8B12,9B12D9,9C11A5)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15480 targets Myeloperoxidase in WB applications and shows reactivity with Human samples.

Antibody clone number

9B12G7,4D8B12,9B12D9,9C11A5

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Myeloperoxidase using a Myeloperoxidase monoclonal antibody (Product # MA5-15480) against a truncated Trx-MPO recombinant protein (1), truncated MBP-MPO (aa1-193) recombinant protein (2) and truncated MPO (aa165-745) human IgG Fc transfected CHO-K1 cell lysate (3).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Myeloperoxidase using a Myeloperoxidase monoclonal antibody (Product # MA5-15480) against a truncated Trx-MPO recombinant protein (1), truncated MBP-MPO (aa1-193) recombinant protein (2) and truncated MPO (aa165-745) human IgG Fc transfected CHO-K1 cell lysate (3).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Myeloperoxidase Monoclonal Antibody (Product # MA5-15480) and 87 kDa, 73 kDa and 60 kDa bands corresponding to MPO was observed across cell lines except K-562 and A-431, also it was observed to decrease upon Retinoic Acid treatment. Membrane enriched extracts (30 µg lysate) of HL-60 (Lane 1), HL-60 treated with Retinoic Acid (1uM for 72 Hours) (Lane 2), K-562 (Lane 3) and A-431 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500 dilution) and detected by chemiluminescence Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).