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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-32512 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Myeloperoxidase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil, spleen or lymph node tissue.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Deficiency in Duox2 activity alleviates ileitis in GPx1- and GPx2-knockout mice without affecting apoptosis incidence in the crypt epithelium.
Nox1 causes ileocolitis in mice deficient in glutathione peroxidase-1 and -2.
Chu FF, Esworthy RS, Doroshow JH, Grasberger H, Donko A, Leto TL, Gao Q, Shen B
Redox biology 2017 Apr;11:144-156
Redox biology 2017 Apr;11:144-156
Nox1 causes ileocolitis in mice deficient in glutathione peroxidase-1 and -2.
Esworthy RS, Kim BW, Chow J, Shen B, Doroshow JH, Chu FF
Free radical biology & medicine 2014 Mar;68:315-25
Free radical biology & medicine 2014 Mar;68:315-25
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Myeloperoxidase Polyclonal Antibody (Product # PA5-32512) and a 60 kDa band corresponding to MPO was observed only in HL-60 and Mouse Bone Marrow. Membrane enriched extracts (30 µg lysate) of HL-60 (Lane 1), K-562 (Lane 2), A-431 (Lane 3), HeLa (Lane 4), tissue extracts of Mouse Spleen (Lane 5) and Mouse Bone Marrow (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Myeloperoxidase using anti-Myeloperoxidase Polyclonal Antibody (Product # PA5-32512) in Lymph Node Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
