PA1054

antibody from Boster Biological Technology

Targeting: MPO

Provider product page for PA1054

Western blot

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [22]
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Validations
Western blot [1]

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Product number: PA1054 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-Myeloperoxidase/MPO Antibody
Antibody type: Polyclonal
Description: Polyclonal antibody for Myeloperoxidase/MPO detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Myeloperoxidase/MPO information: Molecular Weight: 83869 MW; Subcellular Localization: Lysosome.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Vial size: 100μg/vial
Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Handling: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

MPO protein structure - PA1054 shown in red.

Submitted references 17β-estradiol and methylprednisolone association as a therapeutic option to modulate lung inflammation in brain-dead female rats.

Vidal-Dos-Santos M, Anunciação LF, Armstrong-Jr R, Ricardo-da-Silva FY, Ramos IYT, Correia CJ, Moreira LFP, Leuvenink HGD, Breithaupt-Faloppa AC
Frontiers in immunology 2024;15:1375943

Penicilazaphilone C alleviates allergic airway inflammation and improves the immune microenvironment by hindering the NLRP3 inflammasome.

Fu Y, Huang FY, Dai SZ, Wang L, Zhou X, Zheng ZY, Wang CC, Tan GH, Li Q
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Jun;175:116788

Characterization and efficacy of C(60) nano-photosensitive drugs in colorectal cancer treatment.

Fu C, Gong S, Lin L, Bao Y, Li L, Chen Q
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Jul;176:116828

Evaluation of the therapeutic effects of oestradiol on the systemic inflammatory response and on lung injury caused by the occlusion of the proximal descending aorta in male rats.

de Sousa MN, da Anunciação LF, de Freitas PLZ, Ricardo-da-Silva FY, Moreira LFP, Correia CJ, Breithaupt-Faloppa AC
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2023 Sep 7;64(3)

Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration.

Tan J, Zhuo Z, Wang X, Zhang Y, Qian Y, Lin F
International journal of molecular medicine 2023 Nov;52(5)

Biointerface mineralization generates ultraresistant gut microbes as oral biotherapeutics.

Geng Z, Wang X, Wu F, Cao Z, Liu J
Science advances 2023 Mar 15;9(11):eade0997

Butylparaben disordered intestinal homeostasis in Chinese striped-necked turtles (Mauremys sinensis).

Ding L, Wu X, Lin J, Zhang J, Shi H, Hong M, Fang Z
Ecotoxicology and environmental safety 2023 Jun 29;262:115193

Hydroxysafflor yellow A protects against ulcerative colitis via suppressing TLR4/NF-κB signaling pathway.

Feng Z, Zhou P, Wu X, Zhang J, Zhang M
Chemical biology & drug design 2022 Jun;99(6):897-907

Maresin-2 alleviates allergic airway inflammation in mice by inhibiting the activation of NLRP3 inflammasome, Th2 type immune response and oxidative stress.

Yu CX, Shi ZA, Ou GC, Chen XJ, Liu Q, Zeng D, Nie XJ, Chen JJ
Molecular immunology 2022 Jun;146:78-86

HSP60 Regulates Monosodium Urate Crystal-Induced Inflammation by Activating the TLR4-NF-κB-MyD88 Signaling Pathway and Disrupting Mitochondrial Function.

Huang Q, Gao W, Mu H, Qin T, Long F, Ren L, Tang H, Liu J, Zeng M
Oxidative medicine and cellular longevity 2020;2020:8706898

Prevention and Alleviation of Dextran Sulfate Sodium Salt-Induced Inflammatory Bowel Disease in Mice With Bacillus subtilis-Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora.

Zhang X, Tong Y, Lyu X, Wang J, Wang Y, Yang R
Frontiers in microbiology 2020;11:622354

Matrix Metalloproteases-Mediated Cleavage on β-Dystroglycan May Play a Key Role in the Blood-Brain Barrier After Intracerebral Hemorrhage in Rats.

Zhang X, Gu Y, Li P, Jiang A, Sheng X, Jin X, Shi Y, Li G
Medical science monitor : international medical journal of experimental and clinical research 2019 Jan 28;25:794-800

MPO Promoter Polymorphism rs2333227 Enhances Malignant Phenotypes of Colorectal Cancer by Altering the Binding Affinity of AP-2α.

Meng Q, Wu S, Wang Y, Xu J, Sun H, Lu R, Gao N, Yang H, Li X, Tang B, Aschner M, Chen R
Cancer research 2018 May 15;78(10):2760-2769

miR-27a-3p protects against blood-brain barrier disruption and brain injury after intracerebral hemorrhage by targeting endothelial aquaporin-11.

Xi T, Jin F, Zhu Y, Wang J, Tang L, Wang Y, Liebeskind DS, Scalzo F, He Z
The Journal of biological chemistry 2018 Dec 28;293(52):20041-20050

DNase-1 Treatment Exerts Protective Effects in a Rat Model of Intestinal Ischemia-Reperfusion Injury.

Wang S, Xie T, Sun S, Wang K, Liu B, Wu X, Ding W
Scientific reports 2018 Dec 12;8(1):17788

Interleukin-17A Aggravates Middle Ear Injury Induced by Streptococcus pneumoniae through the p38 Mitogen-Activated Protein Kinase Signaling Pathway.

Wang W, Liu W, Liu J, Wang Z, Fan F, Ma Y, Jin C, Xiang Y, Huang Y, Zhang X, Xu W, Yin Y, He Y
Infection and immunity 2017 Oct;85(10)

NAD replenishment with nicotinamide mononucleotide protects blood-brain barrier integrity and attenuates delayed tissue plasminogen activator-induced haemorrhagic transformation after cerebral ischaemia.

Wei CC, Kong YY, Hua X, Li GQ, Zheng SL, Cheng MH, Wang P, Miao CY
British journal of pharmacology 2017 Nov;174(21):3823-3836

The novel exercise-induced hormone irisin protects against neuronal injury via activation of the Akt and ERK1/2 signaling pathways and contributes to the neuroprotection of physical exercise in cerebral ischemia.

Li DJ, Li YH, Yuan HB, Qu LF, Wang P
Metabolism: clinical and experimental 2017 Mar;68:31-42

MicroRNA-126-3p attenuates blood-brain barrier disruption, cerebral edema and neuronal injury following intracerebral hemorrhage by regulating PIK3R2 and Akt.

Xi T, Jin F, Zhu Y, Wang J, Tang L, Wang Y, Liebeskind DS, He Z
Biochemical and biophysical research communications 2017 Dec 9;494(1-2):144-151

The critical role of myeloperoxidase in Streptococcus pneumoniae clearance and tissue damage during mouse acute otitis media.

Xiang Y, Jin C, Wang W, Wang Z, Huang Y, Fan F, Ma Y, Zhang X, Xu W, Yin Y, He Y
Innate immunity 2017 Apr;23(3):296-306

Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway.

Wei CC, Kong YY, Li GQ, Guan YF, Wang P, Miao CY
Scientific reports 2017 Apr 6;7(1):717

Ligustilide ameliorates neuroinflammation and brain injury in focal cerebral ischemia/reperfusion rats: involvement of inhibition of TLR4/peroxiredoxin 6 signaling.

Kuang X, Wang LF, Yu L, Li YJ, Wang YN, He Q, Chen C, Du JR
Free radical biology & medicine 2014 Jun;71:165-175

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Supportive validation

Submitted by: Boster Biological Technology (provider)
Main image
Experimental details: Western blot analysis of Myeloperoxidase/MPO using anti- Myeloperoxidase/MPO antibody (PA1054). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Myeloperoxidase/MPO antigen affinity purified polyclonal antibody (Catalog # PA1054) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Additional image