HPA027824

antibody from Atlas Antibodies

Targeting: P4HA2 C-P4Halpha(II)

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA027824

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA027824, RRID:AB_2672402

Product name

Anti-P4HA2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LDYLSYAVFQLGDLHRALELTRRLLSLDPSHERAG
GNLRYFEQLLEEEREKTLTNQTEAELATPEGIYER
PVDYLPERDVYESLCRGEGVKLTPRRQKRLFCRYH
HGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-P4HA2 antibody HPA027824 (A) shows similar pattern to independent antibody HPA016997 (B).

Antibody #2 product nr

HPA016997

Antibody provider

Atlas Antibodies

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Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human epididymis and skeletal muscle tissues using Anti-P4HA2 antibody. Corresponding P4HA2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human epididymis shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN