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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-53530 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P4HA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LKEYILVEEA KLSKIKSWAN KMEALTSKSA ADAEGYLAHP VNAYKLVKRL NTDWPALEDL VLQDSAAGFI ANLSVQRQFF PTDEDEIGAA KALMRLQDTY RLDPGTISRG ELPGTKYQAM LSVDDCFGM Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 91%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of P4HA2 in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a P4HA2 Polyclonal Antibody (Product # PA5-53530). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of P4HA2 using P4HA2 Polyclonal Antibody (Product # PA5-53530) (A) shows similar pattern to an independent P4HA2 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of P4HA2 in human cell line U-2 OS shows positivity in endoplasmic reticulum & vesicles. Samples were probed using a P4HA2 Polyclonal Antibody (Product # PA5-53530).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA2 in human epididymis and skeletal muscle tissues using P4HA2 Polyclonal Antibody (Product # PA5-53530). Corresponding P4HA2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA2 in human skeletal muscle using P4HA2 Polyclonal Antibody (Product # PA5-53530) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA2 in human epididymis using P4HA2 Polyclonal Antibody (Product # PA5-53530) shows high expression.
