PA5-53530

antibody from Invitrogen Antibodies

Targeting: P4HA2 C-P4Halpha(II)

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-53530

Provider

Invitrogen Antibodies

Product name

P4HA2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LKEYILVEEA KLSKIKSWAN KMEALTSKSA ADAEGYLAHP VNAYKLVKRL NTDWPALEDL VLQDSAAGFI ANLSVQRQFF PTDEDEIGAA KALMRLQDTY RLDPGTISRG ELPGTKYQAM LSVDDCFGM

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of P4HA2 in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a P4HA2 Polyclonal Antibody (Product # PA5-53530). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of P4HA2 using P4HA2 Polyclonal Antibody (Product # PA5-53530) (A) shows similar pattern to an independent P4HA2 Polyclonal Antibody (B).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of P4HA2 in human cell line U-2 OS shows positivity in endoplasmic reticulum & vesicles. Samples were probed using a P4HA2 Polyclonal Antibody (Product # PA5-53530).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of P4HA2 in human epididymis and skeletal muscle tissues using P4HA2 Polyclonal Antibody (Product # PA5-53530). Corresponding P4HA2 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of P4HA2 in human skeletal muscle using P4HA2 Polyclonal Antibody (Product # PA5-53530) shows low expression as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of P4HA2 in human epididymis using P4HA2 Polyclonal Antibody (Product # PA5-53530) shows high expression.