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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- MA5-24599 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P4HA2 Monoclonal Antibody (CL0351)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LKEYILVEEA KLSKIKSWAN KMEALTSKSA ADAEGYLAHP VNAYKLVKRL NTDWPALEDL VLQDSAAGFI ANLSVQRQFF PTDEDEIGAA KALMRLQDTY RLDPGTISRG ELPGTKYQAM LSVDDCFGM Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 91%. Binds to an epitope located within the peptide sequence NTDWPALEDLVLQDS as determined by overlapping synthetic peptides.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL0351
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Targeting HIF-activated collagen prolyl 4-hydroxylase expression disrupts collagen deposition and blocks primary and metastatic uveal melanoma growth.
Kaluz S, Zhang Q, Kuranaga Y, Yang H, Osuka S, Bhattacharya D, Devi NS, Mun J, Wang W, Zhang R, Goodman MM, Grossniklaus HE, Van Meir EG
Oncogene 2021 Aug;40(33):5182-5191
Oncogene 2021 Aug;40(33):5182-5191
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of P4HA2 in Lane1: Marker (kDa), Lane 2: Human cell line U-251 MG. Samples were probed using a P4HA2 Monoclonal Antibody (Product # MA5-24599).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of P4HA2 in extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using P4HA2 Monoclonal Antibody (CL0351) (Product # MA5-24599). Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA2 in human epididymis and skeletal muscle tissues using P4HA2 Monoclonal Antibody (CL0351) (Product # MA5-24599). Corresponding P4HA2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA2 in human epididymis using P4HA2 Monoclonal Antibody (CL0351) (Product # MA5-24599) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA2 in human fallopian tube using P4HA2 Monoclonal Antibody (CL0351) (Product # MA5-24599) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA2 in human small intestine using P4HA2 Monoclonal Antibody (CL0351) (Product # MA5-24599) shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA2 in human skeletal muscle using P4HA2 Monoclonal Antibody (CL0351) (Product # MA5-24599) shows no positivity in striated muscle fibers as expected.
