Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-96280 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P4HA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SHERAGGNLR YFEQLLEEER EKTLTNQTEA ELATPEGIYE RPVDYLPERD VYESLCRGEG VKLTPRRQKR LFCRYHHGNR APQLLIAPFK EEDEW Positive Samples: SKOV3, Mouse brain, Rat brain; Cellular Location: Endoplasmic reticulum lumen
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.49 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Targeting the CBP/β-Catenin Interaction to Suppress Activation of Cancer-Promoting Pancreatic Stellate Cells.
Che M, Kweon SM, Teo JL, Yuan YC, Melstrom LG, Waldron RT, Lugea A, Urrutia RA, Pandol SJ, Lai KKY
Cancers 2020 Jun 5;12(6)
Cancers 2020 Jun 5;12(6)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of P4HA2 in NIH/3T3 cells. Samples were incubated with P4HA2 Polyclonal antibody (Product # PA5-96280) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 CBP/beta-catenin antagonism suppresses protein expression of activation markers of pancreatic stellate cells as assessed by immunoblot. Effect of CBP/beta-catenin antagonist ICG-001 versus control (DMSO) treatment for 72 h of immortalized mouse pancreatic stellate cells (imPSC) on protein expression of activation markers, Acta2 (alpha-SMA) ( A ) and Prolyl 4-hydroxylase (P4HA2) ( B ). Numerical values below protein bands indicate densitometric quantitation normalized to Ponceau S or GAPDH as indicated and then to control (DMSO). Numerical values and associated horizontal marks to the left of protein bands indicate relative position of molecular weight (kDa) markers. (Whole immunoblots are presented in Figure S1 . ).