Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-17072 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSE Monoclonal Antibody (5D3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17072 targets ENO2 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Mouse samples. The MA5-17072 immunogen is purified recombinant fragment of human ENO2 (amino acids: 251-433) expressed in E. Coli. MA5-17072 detects ENO2 which has a predicted molecular weight of approximately 47.3kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5D3
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Propionyl-L-Carnitine is Efficacious in Ulcerative Colitis Through its Action on the Immune Function and Microvasculature.
Scioli MG, Stasi MA, Passeri D, Doldo E, Costanza G, Camerini R, Fociani P, Arcuri G, Lombardo K, Pace S, Borsini F, Orlandi A
Clinical and translational gastroenterology 2014 Mar 20;5(3):e55
Clinical and translational gastroenterology 2014 Mar 20;5(3):e55
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ENO2 using a ENO2 monoclonal antibody (Product # MA5-17072) against a human ENO2 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ENO2 using a ENO2 monoclonal antibody (Product # MA5-17072) against a human ENO2 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ENO2 using ENO2 monoclonal antibody (Product # MA5-17072) in mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ENO2 monoclonal antibody (Product # MA5-17072) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ENO2 monoclonal antibody (Product # MA5-17072) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using ENO2 monoclonal antibody (Product # MA5-17072) (green) and negative control (red).