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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [3]
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- Product number
- PA1-28217 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: IMR cells, Pancreas.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 7 mL
- Storage
- 4° C, do not freeze
Submitted references Enteric neuron density correlates with clinical features of severe gut dysmotility.
Boschetti E, Malagelada C, Accarino A, Malagelada JR, Cogliandro RF, Gori A, Bonora E, Giancola F, Bianco F, Tugnoli V, Clavenzani P, Azpiroz F, Stanghellini V, Sternini C, De Giorgio R
American journal of physiology. Gastrointestinal and liver physiology 2019 Dec 1;317(6):G793-G801
American journal of physiology. Gastrointestinal and liver physiology 2019 Dec 1;317(6):G793-G801
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-NSE Polyclonal Antibody(Product # PA1-28217) and a 47kDa band corresponding to NSE was observed across all tested cell lines and tissues, except SK-BR-3, Mouse Liver and Rat Liver. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), U-87 MG (Lane 2), IMR-32 (Lane 3), SK-BR-3 (Lane 4), Jurkat (Lane 5), Mouse Brain (Lane 6), Rat Brain (Lane 7), Mouse Liver (Lane 8), Rat Liver (Lane 9) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of NSE was performed in human pancreas tissue using NSE Polyclonal Antibody (Product # PA1-28217).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- (Product # PA1-28217) staining NSE in human pancreas by Immunohistochemistry (FFPE-sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
