66150-1-Ig

antibody from Proteintech Group

Targeting: ENO2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

66150-1-Ig

Provider

Proteintech Group

Product name

NSE antibody

Antibody type

Monoclonal

Description

KD/KO validated NSE antibody (Cat. #66150-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat, pig and has been validated for the following applications: IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat, Porcine

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

6F8G3

Vial size

20ul, 150ul

References

Submitted references

Adipose tissue-derived stromal cells enhance glycolytic metabolism in injured nerve cells via the FOXK1-HK2 axis for spinal cord injury repair.

Li F, Li H, Jia Y, Ou L, Fang Y, Pan L, Liu H, Ning B
Journal of translational medicine 2026 Mar 17;24(1)

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Neuronal Subtype-Specific Expression of γ-Enolase: Its Role in Neuronal Differentiation.

Horvat S, Pečar Fonović U, Zidar N, Doljak B, Kos J, Pišlar A
Neuromolecular medicine 2026 Jan 30;28(1):4

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Neural stem cell-derived exosomal PA2G4 induces ANXA2 degradation to promote mitophagy and alleviate neuronal oxidative stress in cerebral ischemia/reperfusion.

Dai M, Lu T, Li J, Yu H
Apoptosis : an international journal on programmed cell death 2026 Feb 20;31(3)

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SNHG18 deficiency reprograms arginine metabolism to foster an immunosuppressive microenvironment in prostate cancer bone metastasis.

Xie Z, Li S, Yang J, Zhang Z, Li S, Lang C, Yang M, Wang B, Yu X, Li Z, Kang Y, Yin C, Yang Q
Cancer letters 2026 Apr 28;644:218326

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ROS-dependent localization of glycolytic enzymes to mitochondria.

Esparza-Moltó PB, Goswami AV, Bozkurt S, Münch C, Newman LE, Moyzis AG, Rojas GR, Guan D, Jones JR, Gage FH, Shadel GS
Redox biology 2025 Oct;86:103812

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Gypenoside XLIX alleviates sepsis-associated encephalopathy by targeting PPAR-α.

Zhao P, Zhang W, Zhou X, Zhao Y, Li A, Sun Y
Experimental neurology 2025 Jan;383:115027

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The important role of the Wnt/β-catenin signaling pathway in small molecules mediated gingival mesenchymal stem cells transdifferentiate into neuron-like cells.

Liang Q, Zhang C, Lv P, Huang Y, Zhao H, Jiang S, Xu W
Archives of oral biology 2025 Jan;169:106115

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ITGA3 promotes pancreatic cancer progression through HIF1α- and c-Myc-driven glycolysis in a collagen I-dependent autocrine manner.

Li R, Ji Q, Fu S, Gu J, Liu D, Wang L, Yuan X, Wen Y, Dai C, Li H
Cancer gene therapy 2025 Feb;32(2):240-253

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Endoplasmic reticulum stress regulates intestinal motility in acute pancreatitis by regulating the expression and phosphorylation of nNOS through IRE1α/XBP1s-PI3K/Akt signaling axis.

Liu H, Cao J, Li X, Zhang C, Pan C, Liang S, Fang H, Liu Y
Cellular signalling 2025 Dec;136:112123

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Trigeminal nerve root compression induced neuroinflammatory response promotes mechanical allodynia through the CGRP/SP-Piezo2 axis via Ca(2+) signaling.

Liao X, Luo Z, Huang F, Wang Y, Zeng Z, Liao W, Ou Y, Wu X, Wang F, Luo D
Cellular & molecular biology letters 2025 Dec 3;31(1):3

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α-Enolase and γ-Enolase Expression in Enriched S- and N-Type SH-SY5Y Cells: Regulatory Role of Cathepsin X.

Horvat S, Zidar N, Kos J, Pišlar A
Molecular neurobiology 2025 Aug;62(8):10006-10019

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The α- to γ-enolase switch: The role and regulation of γ-enolase during oligodendrocyte differentiation.

Horvat S, Fonović UP, Mitrović A, Zidar N, Kos J, Pišlar A
International journal of biological macromolecules 2025 Apr;301:140464

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Histone demethylase PHF8 promotes prostate cancer metastasis via the E2F1-SNAI1 axis.

Wang Z, Tang P, Xiao H, Peng S, Chen J, Wang Y, Xu J, Yan Q, Zhang J, Deng J, Ma Q, Zhu H, Luo W, Zhang D, Wang L, Qin J, Lan W, Jiang J, Liu Q
The Journal of pathology 2024 Sep;264(1):68-79

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A functional variant rs912304 for late-onset T1D risk contributes to islet dysfunction by regulating proinflammatory cytokine-responsive gene STXBP6 expression.

Qian Y, Chen S, Wang Y, Zhang Y, Zhang J, Jiang L, Dai H, Shen M, He Y, Jiang H, Yang T, Fu Q, Xu K
BMC medicine 2024 Sep 4;22(1):357

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Investigation of ENO2 as a promising novel marker for the progression of colorectal cancer with microsatellite instability-high.

Cai J, Yang Y, Zhang L, Fang Y, Zhang Y, Tan M, Zhang J, Tang C, Ren H, Wang L, Xiang G, Xu F, Lan L, Li L, Zheng X
BMC cancer 2024 May 9;24(1):573

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PAX6 promotes neuroendocrine phenotypes of prostate cancer via enhancing MET/STAT5A-mediated chromatin accessibility.

Jing N, Du X, Liang Y, Tao Z, Bao S, Xiao H, Dong B, Gao WQ, Fang YX
Journal of experimental & clinical cancer research : CR 2024 May 15;43(1):144

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Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer.

Wang R, Mi Y, Ni J, Wang Y, Ding L, Ran X, Sun Q, Tan SY, Koeffler HP, Feng N, Chen YQ
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Mar;11(9):e2304939

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Targeting SOX4/PCK2 signaling suppresses neuroendocrine trans-differentiation of castration-resistant prostate cancer.

Jing N, Tao Z, Du X, Wen Z, Gao WQ, Dong B, Fang YX
Biology direct 2024 Jul 16;19(1):56

---

Functional Organization of Glycolytic Metabolon on Mitochondria.

Wang H, Vant J, Wu Y, Sanchez R, Micou ML, Zhang A, Luczak V, Yu SB, Jabbo M, Yoon S, Abushawish AA, Ghassemian M, Griffis E, Hammarlund M, Singharoy A, Pekkurnaz G
bioRxiv : the preprint server for biology 2023 Sep 8;

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HMGCS2 serves as a potential biomarker for inhibition of renal clear cell carcinoma growth.

Mao H, Wang R, Shao F, Zhao M, Tian D, Xia H, Zhao Y
Scientific reports 2023 Sep 5;13(1):14629

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MYC is a regulator of androgen receptor inhibition-induced metabolic requirements in prostate cancer.

Crowell PD, Giafaglione JM, Jones AE, Nunley NM, Hashimoto T, Delcourt AML, Petcherski A, Agrawal R, Bernard MJ, Diaz JA, Heering KY, Huang RR, Low JY, Matulionis N, Navone NM, Ye H, Zoubeidi A, Christofk HR, Rettig MB, Reiter RE, Haffner MC, Boutros PC, Shirihai OS, Divakaruni AS, Goldstein AS
Cell reports 2023 Oct 31;42(10):113221

---

Prostate lineage-specific metabolism governs luminal differentiation and response to antiandrogen treatment.

Giafaglione JM, Crowell PD, Delcourt AML, Hashimoto T, Ha SM, Atmakuri A, Nunley NM, Dang RMA, Tian M, Diaz JA, Tika E, Payne MC, Burkhart DL, Li D, Navone NM, Corey E, Nelson PS, Lin NYC, Blanpain C, Ellis L, Boutros PC, Goldstein AS
Nature cell biology 2023 Dec;25(12):1821-1832

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Integrated Analysis of the Role of Enolase 2 in Clear Cell Renal Cell Carcinoma.

Pan J, Jin Y, Xu X, Wei W, Pan H
Disease markers 2022;2022:6539203

---

Supplementation with dietary omega-3 PUFA mitigates fetal brain inflammation and mitochondrial damage caused by high doses of sodium nitrite in maternal rats.

Sun J, Zhang W
PloS one 2022;17(3):e0266084

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Biliverdin modulates the long non-coding RNA H19/microRNA-181b-5p/endothelial cell specific molecule 1 axis to alleviate cerebral ischemia reperfusion injury.

Li J, Jiang H, Peng P, Zhang Q, Bai W, Yang Y, Huo S, Zhou G, Shao J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022 Sep;153:113455

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POU6F1 cooperates with RORA to suppress the proliferation of lung adenocarcinoma by downregulation HIF1A signaling pathway.

Xiao W, Geng W, Zhou M, Xu J, Wang S, Huang Q, Sun Y, Li Y, Yang G, Jin Y
Cell death & disease 2022 May 3;13(5):427

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Extracellular ATP and cAMP signaling promote Piezo2-dependent mechanical allodynia after trigeminal nerve compression injury.

Luo Z, Liao X, Luo L, Fan Q, Zhang X, Guo Y, Wang F, Ye Z, Luo D
Journal of neurochemistry 2022 Feb;160(3):376-391

---

Tetrandrine alleviates inflammation and neuron apoptosis in experimental traumatic brain injury by regulating the IRE1α/JNK/CHOP signal pathway.

Liu H, He S, Li C, Wang J, Zou Q, Liao Y, Chen R
Brain and behavior 2022 Dec;12(12):e2786

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Neuron-specific enolase promotes stem cell-like characteristics of small-cell lung cancer by downregulating NBL1 and activating the BMP2/Smad/ID1 pathway.

Lu L, Zha Z, Zhang P, Wang P, Liu X, Fang X, Weng C, Li B, Mao H, Wang L, Guan M, Wu Y, Xu Z, Liu Z, Liu G
Oncogenesis 2022 Apr 29;11(1):21

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Reciprocal regulation of LOXL2 and HIF1α drives the Warburg effect to support pancreatic cancer aggressiveness.

Li R, Li H, Zhu L, Zhang X, Liu D, Li Q, Ni B, Hu L, Zhang Z, Zhang Y, Wang X, Jiang SH
Cell death & disease 2021 Nov 26;12(12):1106

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Effects of morphine preconditioning on TRPV1 sensitization and ERK1/2 phosphorylation induced by TGFβ1 in neurocytes.

Ma ZX, Wang S
Neuroreport 2021 Mar 24;32(5):339-344

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Annexin A7 induction of neuronal apoptosis via effect on glutamate release in a rat model of subarachnoid hemorrhage.

Lin QS, Wang WX, Lin YX, Lin ZY, Yu LH, Kang Y, Kang DZ
Journal of neurosurgery 2020 Mar 1;132(3):777-787

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Sema7A, a brain immune regulator, regulates seizure activity in PTZ-kindled epileptic rats.

Deng J, Xu T, Yang J, Zhang KM, Li Q, Yu XY, Li R, Fu J, Jiang Q, Ma JX, Chen YM
CNS neuroscience & therapeutics 2020 Jan;26(1):101-116

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In Vitro Neural Differentiation of Bone Marrow Mesenchymal Stem Cells Carrying the FTH1 Reporter Gene and Detection with MRI.

Mu T, Qin Y, Liu B, He X, Liao Y, Sun J, Qiu J, Li X, Zhong Y, Cai J
BioMed research international 2018;2018:1978602

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The NSE antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human NSE. This antibody recognizes human,mouse, rat antigen. The NSE antibody has been validated for the following applications: ELISA, WB analysis.

Appendix

66150-1-Ig.pdf

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