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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX113428 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113428, RRID:AB_1950201
- Product name
- NSE antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE GTX113428 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) 10% SDS PAGE GTX113428 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NSE antibody [C1C3] detects NSE protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NSE protein stained by NSE antibody [C1C3] (GTX113428) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NSE antibody [C1C3] detects NSE protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [C1C3] (GTX113428) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
