PAB12508

antibody from Abnova Corporation

Targeting: ENO2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12508

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12508, RRID:AB_10560347

Product name

ENO2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against full length recombinant ENO2.

Storage

Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury.

Begaz T, Kyriacou DN, Segal J, Bazarian JJ
Journal of neurotrauma 2006 Aug;23(8):1201-10

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Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.

Shaw G, Morse S, Ararat M, Graham FL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2002 Jun;16(8):869-71

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot of ENO2 in rat spinal cord probed with ENO2 polyclonal antibodyCat # PAB12508 ). The antibody stains a single sharp band corresponing to NSE at about 47kDa.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The red channel shows staining which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker to ENO2 using ENO2 polyclonal antibody (Cat # PAB12508). This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.

Validation comment

Immunofluorescence