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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [22]
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- Immunohistochemistry [1]
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- Product number
- A02930 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-NSE/ENO2 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for NSE detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Biofunctionalized self-assembly of peptide amphiphile induces the differentiation of bone marrow mesenchymal stem cells into neural cells.
The impact of bone marrow-derived mesenchymal stem cells on neovascularisation in rats with brain injury.
Exercise Mitigates Alcohol Induced Endoplasmic Reticulum Stress Mediated Cognitive Impairment through ATF6-Herp Signaling.
Neuroprotective Effect of the Ginsenoside Rg1 on Cerebral Ischemic Injury In Vivo and In Vitro Is Mediated by PPARγ-Regulated Antioxidative and Anti-Inflammatory Pathways.
In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats.
Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene.
Effects of transforming growth factor-β2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells.
Neuroprotective effect of Atractylodes macrocephalaon polysaccharides in vitro on neuronal apoptosis induced by hypoxia.
Mdivi-1 prevents apoptosis induced by ischemia-reperfusion injury in primary hippocampal cells via inhibition of reactive oxygen species-activated mitochondrial pathway.
Amyloid beta-derived diffusible ligands (ADDLs) induce abnormal expression of insulin receptors in rat hippocampal neurons.
Neuroprotective effects of oxysophocarpine on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion.
β-elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells.
Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury.
Wnt1 from cochlear schwann cells enhances neuronal differentiation of transplanted neural stem cells in a rat spiral ganglion neuron degeneration model.
Expression pattern of sorting Nexin 25 in temporal lobe epilepsy: a study on patients and pilocarpine-induced rats.
Injury of cortical neurons is caused by the advanced glycation end products-mediated pathway.
Brain targeting and toxicity study of odorranalectin-conjugated nanoparticles following intranasal administration.
Modulation of migration and Ca2+ signaling in retinal pigment epithelium cells by recombinant human CTGF.
The effect of 3-hydroxybutyrate methyl ester on learning and memory in mice.
Ex vivo expansion, adipogenesis and neurogenesis of cryopreserved human bone marrow mesenchymal stem cells.
The structural characteristics and expression of neuropeptides in the esophagus of patients with congenital esophageal atresia and tracheoesophageal fistula.
UEA I-bearing nanoparticles for brain delivery following intranasal administration.
Ruan H, Xiao R, Jiang X, Zhao B, Wu K, Shao Z, Zhang Z, Duan H, Song Y
Molecular and cellular biochemistry 2019 Jan;450(1-2):199-207
Molecular and cellular biochemistry 2019 Jan;450(1-2):199-207
The impact of bone marrow-derived mesenchymal stem cells on neovascularisation in rats with brain injury.
Hu W, Jiang J, Yang F, Liu J
Folia neuropathologica 2018;56(2):112-123
Folia neuropathologica 2018;56(2):112-123
Exercise Mitigates Alcohol Induced Endoplasmic Reticulum Stress Mediated Cognitive Impairment through ATF6-Herp Signaling.
George AK, Behera J, Kelly KE, Mondal NK, Richardson KP, Tyagi N
Scientific reports 2018 Mar 26;8(1):5158
Scientific reports 2018 Mar 26;8(1):5158
Neuroprotective Effect of the Ginsenoside Rg1 on Cerebral Ischemic Injury In Vivo and In Vitro Is Mediated by PPARγ-Regulated Antioxidative and Anti-Inflammatory Pathways.
Li Y, Guan Y, Wang Y, Yu CL, Zhai FG, Guan LX
Evidence-based complementary and alternative medicine : eCAM 2017;2017:7842082
Evidence-based complementary and alternative medicine : eCAM 2017;2017:7842082
In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats.
Ye Y, Peng YR, Hu SQ, Yan XL, Chen J, Xu T
Frontiers in neurology 2016;7:183
Frontiers in neurology 2016;7:183
Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene.
Liu Q, Cheng G, Wang Z, Zhan S, Xiong B, Zhao X
In vitro cellular & developmental biology. Animal 2015 Mar;51(3):319-27
In vitro cellular & developmental biology. Animal 2015 Mar;51(3):319-27
Effects of transforming growth factor-β2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells.
Wu Y, Chen W, Guo M, He Q, Hu Y
International journal of clinical and experimental pathology 2014;7(8):4827-36
International journal of clinical and experimental pathology 2014;7(8):4827-36
Neuroprotective effect of Atractylodes macrocephalaon polysaccharides in vitro on neuronal apoptosis induced by hypoxia.
Hu WX, Xiang Q, Wen Z, He D, Wu XM, Hu GZ
Molecular medicine reports 2014 Jun;9(6):2573-81
Molecular medicine reports 2014 Jun;9(6):2573-81
Mdivi-1 prevents apoptosis induced by ischemia-reperfusion injury in primary hippocampal cells via inhibition of reactive oxygen species-activated mitochondrial pathway.
Wang J, Wang P, Li S, Wang S, Li Y, Liang N, Wang M
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 2014 Jul;23(6):1491-9
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 2014 Jul;23(6):1491-9
Amyloid beta-derived diffusible ligands (ADDLs) induce abnormal expression of insulin receptors in rat hippocampal neurons.
Liu X, Teng Z, Cui C, Wang R, Liu M, Zhang Y
Journal of molecular neuroscience : MN 2014 Jan;52(1):124-30
Journal of molecular neuroscience : MN 2014 Jan;52(1):124-30
Neuroprotective effects of oxysophocarpine on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion.
Zhu QL, Li YX, Zhou R, Ma NT, Chang RY, Wang TF, Zhang Y, Chen XP, Hao YJ, Jin SJ, Ma L, Du J, Sun T, Yu JQ
Pharmaceutical biology 2014 Aug;52(8):1052-9
Pharmaceutical biology 2014 Aug;52(8):1052-9
β-elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells.
Zhu TZ, Li XM, Luo LH, Song ZQ, Gao X, Li ZQ, Su JY, Liang GB
International journal of oncology 2014 Aug;45(2):699-709
International journal of oncology 2014 Aug;45(2):699-709
Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury.
Liu G, Zhao L, Wang T, Zhang M, Pei H
Neural regeneration research 2014 Aug 1;9(15):1437-45
Neural regeneration research 2014 Aug 1;9(15):1437-45
Wnt1 from cochlear schwann cells enhances neuronal differentiation of transplanted neural stem cells in a rat spiral ganglion neuron degeneration model.
He Y, Zhang PZ, Sun D, Mi WJ, Zhang XY, Cui Y, Jiang XW, Mao XB, Qiu JH
Cell transplantation 2014 Apr;23(6):747-60
Cell transplantation 2014 Apr;23(6):747-60
Expression pattern of sorting Nexin 25 in temporal lobe epilepsy: a study on patients and pilocarpine-induced rats.
Du Y, Zou Y, Yu W, Shi R, Zhang M, Yang W, Duan J, Deng Y, Wang X, Lü Y
Brain research 2013 May 6;1509:79-85
Brain research 2013 May 6;1509:79-85
Injury of cortical neurons is caused by the advanced glycation end products-mediated pathway.
Xing Y, Zhang X, Song X, Lv Z, Hou L, Li F
Neural regeneration research 2013 Apr 5;8(10):909-15
Neural regeneration research 2013 Apr 5;8(10):909-15
Brain targeting and toxicity study of odorranalectin-conjugated nanoparticles following intranasal administration.
Wen Z, Yan Z, He R, Pang Z, Guo L, Qian Y, Jiang X, Fang L
Drug delivery 2011 Nov;18(8):555-61
Drug delivery 2011 Nov;18(8):555-61
Modulation of migration and Ca2+ signaling in retinal pigment epithelium cells by recombinant human CTGF.
Guo CM, Wang YS, Hu D, Han QH, Wang JB, Hou X, Hui YN
Current eye research 2009 Oct;34(10):852-62
Current eye research 2009 Oct;34(10):852-62
The effect of 3-hydroxybutyrate methyl ester on learning and memory in mice.
Zou XH, Li HM, Wang S, Leski M, Yao YC, Yang XD, Huang QJ, Chen GQ
Biomaterials 2009 Mar;30(8):1532-41
Biomaterials 2009 Mar;30(8):1532-41
Ex vivo expansion, adipogenesis and neurogenesis of cryopreserved human bone marrow mesenchymal stem cells.
Xiang Y, Zheng Q, Jia B, Huang G, Xie C, Pan J, Wang J
Cell biology international 2007 May;31(5):444-50
Cell biology international 2007 May;31(5):444-50
The structural characteristics and expression of neuropeptides in the esophagus of patients with congenital esophageal atresia and tracheoesophageal fistula.
Li K, Zheng S, Xiao X, Wang Q, Zhou Y, Chen L
Journal of pediatric surgery 2007 Aug;42(8):1433-8
Journal of pediatric surgery 2007 Aug;42(8):1433-8
UEA I-bearing nanoparticles for brain delivery following intranasal administration.
Gao X, Chen J, Tao W, Zhu J, Zhang Q, Chen H, Jiang X
International journal of pharmaceutics 2007 Aug 1;340(1-2):207-15
International journal of pharmaceutics 2007 Aug 1;340(1-2):207-15
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of NSE using anti-NSE antibody (A02930).NSE was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NSE Antibody (A02930) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
