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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 66150-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSE Monoclonal Antibody (6F8G3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6F8G3
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- U-251 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of NSE antibody (66150-1-IG; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE transfected U-251 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- U-251 cells were subjected to SDS PAGE followed by western blot with 66150-1-IG ( NSE antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C6 cells were subjected to SDS PAGE followed by western blot with 66150-1-Ig (NSE antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SH-SY5Y cells using 66150-1-IG (NSE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 66150-1-IG ( NSE antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 66150-1-IG ( NSE antibody at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 µg Anti-Human NSE (Product # 66150-1-IG, Clone:6F8G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
