- PA1-46203 - Invitrogen Antibodies ENO2 antibody

Submitted references The dual anti-inflammatory and antioxidant activities of natural honey promote cell proliferation and neural regeneration in a rat model of colitis.

Nooh HZ, Nour-Eldien NM
Acta histochemica 2016 Jul;118(6):588-595

Valproic acid, a histone deacetylase inhibitor, decreases proliferation of and induces specific neurogenic differentiation of canine adipose tissue-derived stem cells.

Kurihara Y, Suzuki T, Sakaue M, Murayama O, Miyazaki Y, Onuki A, Aoki T, Saito M, Fujii Y, Hisasue M, Tanaka K, Takizawa T
The Journal of veterinary medical science 2014 Jan;76(1):15-23

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NSE in rat brain, rat spinal cord, mouse brain, mouse spinal cord, NIH-3T3, HEK293, HeLa, SH-SY5Y, and C6 cells. Samples were incubated in NSE polyclonal antibody (Product # PA1-46203 using a dilution of 1:5,000. Target antibody in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47 kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of NSE was achieved by transfecting HEK-293 cells with NSE specific validated siRNAs (Silencer® select Product # s4685 ). Western blot analysis (Fig. a) was performed using whole cell extracts from the NSE knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with NSE Polyclonal Antibody (Product # PA1-46203, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NSE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), HEK-293 (Lane 2), PC-12 (Lane 3), T98G (Lane 4), HeLa (Lane 5) and U-87 MG (Lane 6). The blot was probed with Anti-NSE Polyclonal Antibody (Product # PA1-46203, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 47 kDa band corresponding to NSE was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NSE in rat brain, rat spinal cord, mouse brain, mouse spinal cord, NIH-3T3, HEK293, HeLa, SH-SY5Y, and C6 cells. Samples were incubated in NSE polyclonal antibody (Product # PA1-46203 using a dilution of 1:5,000. Target antibody in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47 kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NSE in rat spinal cord. Samples were incubated in NSE polyclonal antibody (Product # PA1-46203). The antibody stains a single sharp band corresponing to NSE at about 47 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NSE in extracts from mouse brain. Samples were incubated in NSE polyclonal antibody (Product # PA1-46203) using a dilution of 1:100.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NSE in 0.05 mg/mL Human Brain lysate. Samples were incubated in NSE polyclonal antibody (Product # PA1-46203). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of NSE was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NSE Polyclonal Antibody (Product # PA1-46203) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and cell membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of NSE in human embryonic kidney cells line 293, which express many neuronal proteins (1). Samples were incubated in NSE polyclonal antibody (Product # PA1-46203). The red channel shows staining which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker to UCHL1. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of NSE in mixed cortical neuron-glial cell culture from E20 rat. Samples were incubated in NSE polyclonal antibody (Product # PA1-46203) using a dilution of 1:500. Neuron specific enolase in red and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. the NSE antibody labels protein expressed in neuronal cells, while the GFAP antibody stains intermediate filaments in astrocytic and certain other glial cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of NSE in 3T3 cells. Samples were incubated in NSE polyclonal antibody (Product # PA1-46203) followed by Alexa Fluor 488-conjugated Goat to rabbit IgG secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).

PA1-46203

Antibody data