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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- Ab118776 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- NSE Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human NSE Antibody, Monoclonal (C10), could be used for WB, IHC, ICC, IF and so on.Application:WB: 0.5-2µg/mLIHC: 5-30µg/mLICC/IF: 5-30µg/mLProtein Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 2-285- Antibody clone number
- C10
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- NSE Mouse mAb (Ab118776) - Western Blot All lanes: NSE Mouse mAb (Ab118776) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 16.4 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- NSE Mouse mAb (Ab118776) - IF/ICC FITC staining on IF Samples: Human Hep G2 cells Primary Ab: 20µg/mL NSE Mouse mAb (Ab118776) Second Ab: 1µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- NSE Mouse mAb (Ab118776) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- NSE Mouse mAb (Ab118776) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
